苍耳子水提取液对小鼠肝毒性“量-时-毒”关系研究Study on "dose-time-toxicity" relationship on hepatotoxicity caused by water extract of Xanthium in mice
汪永忠;洪燕;李钰馨;韩燕全;
摘要(Abstract)：
目的:考察苍耳子水提物单次给予对小鼠肝毒性的"量-时-毒"关系。方法:将小鼠按不同时间点和不同剂量分组,观察小鼠急性肝毒性反应,检测血清谷氨酸氨基转移酶(ALT)、谷草氨酸氨基转移酶(AST)水平,计算肝体比值,光学显微镜下观察肝组织病理形态变化。结果:"时-毒"关系研究表明:制备4.0 g/m L苍耳子水提液,取小鼠按不同时间点分组,按101.14 g/kg剂量小鼠灌胃给药一次,分别于给药后各时间点取血,小鼠血清ALT,AST均在给药1 h后明显升高,给药后8 h达到高峰,24 h后恢复近正常值;肝体比值增大,肝组织病理损伤严重;"量-毒"关系研究表明:小鼠单次灌胃给药不同剂量(101.14 g/kg、83.312 g/kg、66.650 g/kg、53.320 g/kg、42.656 g/kg、空白组同体积蒸馏水)。苍耳子水提液对肝组织产生明显损伤,随剂量增大,血清ALT,AST值显著升高。肝体比值增大,肝组织病理损伤严重。结论:小鼠单次灌胃给药苍耳子水提取液可造成明显急性肝损伤,并呈现一定"量-时-毒"关系。
关键词(KeyWords)： 苍耳子水提液;肝毒性;时毒;量毒
基金项目(Foundation): 国家中医药管理局中药炮制技术传承基地项目
作者(Author): 汪永忠;洪燕;李钰馨;韩燕全;
Email:
DOI:
参考文献(References)：
- [1]Hsu F L,Chen Y C,Cheng J T.Caffeic acid as active principle from the fruit of Xanthium strumarium to lower plasma glucose in diabetic rats[J].Planta Medica,2000(66):228-230.
- [2]Hsu H Y,Chen Y P,Sheu S J,et al.Oriental materia medica a concise guide[M].Taipei:Modern Drug Weekly of Taiwan,1985:277.
- [3]Kellerman T S,Coetzer J A W,Naude T W.Plant poisonings and mycotoxicoses of livestock in Southern Africa[M].Cape Town:Oxford University Press,1988.
- [4]Wu B,Cao M,Liu S M,et al.Experiment study on hepatotoxicity induced by the water extract of cocklebur fruit in rats[J].Adverse Drug Reactions Journal,2010,12(6),381-386.
- [5]Xue L M,Zhang Q Y,Han P,et al.Hepatotoxic constituents and toxicological mechanism of Xanthium strumarium L.fruits[J].J Ethnopharmacol,2014,152(2):272-282.
- [6]曲莉颖,权锦花,孙彤,等.105例苍耳子不良反应文献分析[J].辽宁中医药大学学报,2015,17(2):127-129.
- [7]Nikles S,Heuberger H,Hilsdorf E,et al.Influence of processing on the content of toxic carboxyatractyloside and atractyloside and the microbiological status of xanthium sibiricum fruits(Canger zi)[J].Planta Med,2015,81(12/13):1 213-1 220.